JAMP (JNK1/MAPK8 associated membrane protein)

Fri, 02/13/2015 - 14:21


JAMP is a seven-transmembrane protein that is a regulator of JNK/MAPK8 activity in response to various stress stimuli. This regulation is part of a broader collective and coordinated response to clearing misfolded proteins from the ER. JAMP facilitates the degradation of misfolded ER luminal proteins via recruitment of the ERAD (endoplasmic reticulum-associated degradation system). Aside from its interactions with MAPK8, JAMP also interacts with Ring finger protein 5 (RNF5), as well as many regulatory proteins in the ERAD such as AMFR/GP78, CANX, PSMC1/PSMC2 and PSMC5-8. JAMP undergoes a post-translational modification after ubiquitination by RNF5 in a UBE2N-dependent manner, which ultimately decreases its association with the proteasome and ERAD. JAMP is expressed in the endoplasmic reticulum (ER) of cells within many tissues such as brain, spleen, thymus, liver, kidney and testis; medulloblastomas also exhibit elevated JAMP expression.

jamp antibody

Immunohistochemistry-Paraffin: JAMP Antibody

Kadoya et al from the Burnham Institute originally reported that JAMP regulates JNK-1 activity (1). Using both genetic and biochemical approaches, the same group also demonstrated in related follow-up studies that JAMP optimizes the ERAD to protect cells from unfolded proteins; this has various implications for disease models (2). Roy’s laboratory employed a novel gel-free proteomics strategy identify partners of the B2-adrenergic receptor (B2AR) (3). They believe that RNF5 modulates the turnover of G protein-coupled receptors through the ubiquitination of JAMP and subsequent inhibition of proper and required proteasome recruitment. Some interesting studies from Columbia University evaluated the role of JNK in hepatic fibrosis as compared to its already known role in disease processes such as toxic, metabolic, and autoimmune liver injury (4). This group discovered that JNK is involved in hepatic stellate cell (HSC) activation and fibrogenesis.

Novus Biologicals offers JAMP reagents for your research needs including:

PMIDs

  1. 16166642
  2. 18784250
  3. 23798571
  4. 19782079

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