JAM-C Antibody (208212) [Unconjugated]

Images

 
Human platelets were stained with anti-human CD41 FITC-conjugated antibody and either (A) Mouse Anti-Human JAM‑C Monoclonal Antibody (Catalog # MAB11891) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
208212
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

JAM-C Antibody (208212) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-C
Val32-Asn241(Ala149Pro)
Accession # Q9BX67
Specificity
Detects human JAM-C in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-C Antibody (208212) [Unconjugated]

  • CD323
  • JAM-2
  • JAM3
  • JAMC
  • JAM-C
  • JAM-CFLJ14529
  • junctional adhesion molecule 3JAM-3
  • junctional adhesion molecule C

Background

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3‑5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3‑5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826.
  4. Liang, T. et al. (2002) J. Immunol. 168:1618.
  5. Johnson-Leger, C. et al. (2002) Blood 100:25793.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
  8. Phillips, H.M. et al. (2002) Genomics 79:475.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1103
Species: Hu
Applications: WB, IHC, ICC
AF1074
Species: Hu, Mu
Applications: WB, Simple Western, IHC, Neut
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC
NB110-89474
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
NBP2-14286
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-85047
Species: Hu, Po
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KO
AF1103
Species: Hu
Applications: WB, IHC, ICC
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-02259
Species: Hu, Po
Applications: Flow, ICC/IF, ICC
NBP1-87402
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NBP1-88861
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-88192
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for JAM-C Antibody (MAB11891) (0)

There are no publications for JAM-C Antibody (MAB11891).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for JAM-C Antibody (MAB11891) (0)

There are no reviews for JAM-C Antibody (MAB11891). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for JAM-C Antibody (MAB11891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Diseases for JAM-C Antibody (MAB11891)

Discover more about diseases related to JAM-C Antibody (MAB11891).
 

Pathways for JAM-C Antibody (MAB11891)

View related products by pathway.

PTMs for JAM-C Antibody (MAB11891)

Learn more about PTMs related to JAM-C Antibody (MAB11891).

Blogs on JAM-C

There are no specific blogs for JAM-C, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ICAM-1/CD54
796-IC-050
JAM-C
1213-J3-050

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our JAM-C Antibody (208212) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Uniprot