ITM2C Antibody

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITM2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2C at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ITM2C Antibody Summary

• Immunogen: E. coli-derived recombinant human ITM2C
  Ala86-Val267
  Accession # Q9NQX7
• Specificity: Detects human ITM2C in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ITM2A and rhITM2B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITM2C
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ITM2C Antibody

• BRI3
• BRI3E25C
• BRICD2C
• BRICHOS domain containing 2C
• Cerebral protein 14
• E25
• hRPC.1050_D_4
• integral membrane protein 2C
• integral membrane protein 3
• ITM2C
• ITM3
• Transmembrane protein BRI3

Background

ITM2C (Integral membrane protein 2C; also BRI3 and cerebral protein 14) is a 36‑38 kDa member of the BRI₃ family of proteins. It is found on neurons and appears to interact with APP, where ITM2C binding blocks proteolytic processing of APP, plus SCG10, where ITM2C binding blocks the microtubule-destabilizing activity of SCG10, thus inhibiting neurite outgrowth. Human ITM2C is a 267 amino acid (aa) type II transmembrane glycoprotein. Proteolytic cleavage by furin at Arg242Gly243 may create a truncated 34‑35 kDa ITM2C plus a C-terminal 4 kDa active peptide (aa 243‑267). The ECD (aa 76‑267) contains one BRICHOS domain (aa 136‑230). ITM2C may exist as a dimer. There are two splice variants, one that shows a deletion of aa 41‑87, and a second that shows a deletion of aa 151‑187. Over aa 86‑267, human ITM2C shares 96% aa identity with mouse ITM2C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITM2C
• Uniprot:
  • Q9NQX7(Human)