ITM2B Antibody

Images

 
Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963] - Human small intestine tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody ...read more
Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ITM2B Antibody Summary

Immunogen
Peptide with sequence C-HDKETYKLQRRETIK corresponding to internal region according to NP_068839.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITM2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:8000
Application Notes
Western blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1).
Control
ITM2B Knockout HeLa Cell Lysate
Publications
Read Publication using NBP1-36963.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ITM2B Antibody

  • ABRI
  • ABri/ADan amyloid peptide
  • BRI
  • BRI2
  • BRICD2B
  • BRICHOS domain containing 2B
  • BRIFBD
  • E25B
  • E3-16
  • FBD
  • integral membrane protein 2B
  • ITM2B
  • Protein E25B
  • Transmembrane protein BRI

Background

ITM2B is a type II membrane protein. It is expressed in brain and in other tissues. Defects in ITM2B seem to be associated with familial British dementia (FBD) and familial Danish dementia (FDD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ITM2B Antibody (NBP1-36963)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ITM2B Antibody (NBP1-36963) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ITM2B Antibody (NBP1-36963)

Discover related pathways, diseases and genes to ITM2B Antibody (NBP1-36963). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol ITM2B
Entrez
Uniprot