Isocitrate Dehydrogenase 1/IDH1 Antibody (25H10) - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (25H10) [NBP2-59427] - Lane 1: HepG2 cell lysates, Lane 2: A549 cell lysates, Lane 3: HeLa cell lysates, Lane 4: NIH3T3 cell lysates, Lane 5: Jurkat cell lysates, Lane 6: K562 cell lysates, Lane 7: 293T cell lysates, Lane 8: MCF7 cell lysates, Lane 9: LnCaP cell lysates

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (25H10) [NBP2-59427] - Analysis of IDH1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IDH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 25H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Isocitrate Dehydrogenase 1/IDH1 Antibody (25H10) - BSA Free Summary

• Immunogen: Recombinant human Isocitrate Dehydrogenase 1/IDH1 (1-414aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (25H10) - BSA Free

• Cytosolic NADP-isocitrate dehydrogenase
• EC 1.1.1.42
• IDCD
• IDH
• IDH1
• IDP
• IDPC
• isocitrate dehydrogenase [NADP] cytoplasmic
• isocitrate dehydrogenase 1 (NADP+), soluble
• Isocitrate Dehydrogenase 1
• NADP(+)-specific ICDH
• NADP-dependent isocitrate dehydrogenase, cytosolic
• NADP-dependent isocitrate dehydrogenase, peroxisomal
• Oxalosuccinate decarboxylase
• PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH1