Islet-1 Antibody

Images

 
Western Blot: Islet-1 Antibody [NBP2-14999] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody [NBP2-14999] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Frozen: Islet-1 Antibody [NBP2-14999] - Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: ...read more
Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: Islet-1 Antibody [NBP2-14999] - Paraffin-embedded rat fore brain. Islet 1 antibody dilution: 1:100.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Fi, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.88 mg/ml

Order Details

Islet-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary.
Predicted Species
Fish (97%), Zebrafish (97%), Bovine (100%), Chicken (99%), Xenopus (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ISL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%), Japanese medaka (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Islet-1 Antibody

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ISL1
Entrez
Uniprot