IRF7 Antibody - BSA Free

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Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody [NBP3-06987] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with NBP3-06987 at 20ug/mL, followed by goat anti-rabbit IgG ...read more
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for ...read more
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 10ug of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: NBP3-06987 (0.5 ug/mL) and beta-actin, 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat ...read more
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded human colon tissue using NBP3-06987 at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hr ...read more
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for ...read more
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded human liver tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h ...read more
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using NBP3-06987 at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

IRF7 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.
Specificity
Several isoforms of IRF7 are known to exist. Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). NBP3-06987 can detect human isoforms and mouse, and possibly can detect rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRF7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2-5 ug/mL
  • Immunohistochemistry-Paraffin 2-5 ug/mL
  • Knockout Validated
  • Western Blot 0.5-1 ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IRF7 Antibody - BSA Free

  • interferon regulatory factor 7
  • interferon regulatory factor-7H
  • IRF-7
  • IRF7A
  • IRF-7H

Background

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRF7 Antibody (NBP3-06987) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IRF7 Products

Research Areas for IRF7 Antibody (NBP3-06987)

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Blogs on IRF7.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

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Bioinformatics

Gene Symbol IRF7