IRF5 Antibody

Images

 
Western Blot: IRF5 Antibody [NB100-1092] - Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, Flow, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IRF5 Antibody Summary

Immunogen
Peptide with sequence C-QGPWPMHPAGMQ corresponding to C-Terminus according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1.
Specificity
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1:10-1:1000
  • Peptide ELISA Detection limit 1:32000
Application Notes
Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1.
. IHC: An anonymous customer has reported nuclear staining in human cervix.
ChIP:his antibody has been successfully used in human monocyte-derived dendritic cells.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
IRF5 Lysate (NBL1-12034)
Publications
Read Publications using
NB100-1092 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF5 Antibody

  • IBD14
  • interferon regulatory factor 5
  • IRF5
  • IRF-5
  • SLEB10

Background

Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix-turn-helix DNA-binding motif and mediate virus- and interferon (IFN)-induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115-amino acid N-terminal DNA-binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF5 Antibody (NB100-1092)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ChIP, FLOW.


Filter By Application
ChIP
(1)
FLOW
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB100-1092 Applications Species
Li D, De S, Li D et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep 2016 Aug 02 [PMID: 27481535]
Hanke ML, Angle A, Kielian T. MyD88-Dependent Signaling Influences Fibrosis and Alternative Macrophage Activation during Staphylococcus aureus Biofilm Infection plos One 2012 [PMID: 22879997] (FLOW, Mouse) FLOW Mouse
Krausgruber T, Saliba D, Ryzhakov G, Lanfrancotti A, Blazek K, Udalova IA. IRF5 is required for late-phase TNF secretion by human dendritic cells. Blood;115(22):4421-30. 2010 Jun 3. [PMID: 20237317] (ChIP) ChIP
Hu G, Barnes BJ. IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway. J Biol Chem;284(5):2767-77. 2009 Jan 30. [PMID: 19028697]
Ye L, Peng JS, Wang X, Wang YJ, Luo GX, Ho WZ. Methamphetamine enhances Hepatitis C virus replication in human hepatocytes. J Viral Hepat;15(4):261-70. 2008 Apr. [PMID: 18307590]
Graham RR et al. Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus. Proc Natl Acad Sci U S A;104(16):6758-63. 2007 Apr 17. [PMID: 17412832]
Ning S, Huye LE, Pagano JS. Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit. J Virol;79(18):11671-6. 2005 Sep. [PMID: 16140744]
Barnes BJ, Moore PA, Pitha PM. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes. J Biol Chem 2001 Jun 29 [PMID: 11303025]

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IRF5
Entrez
Uniprot