IRF5 Antibody (6L2B5)

Western Blot: IRF5 Antibody (6L2B5) [NBP3-15343] - Western blot analysis of extracts of various cell lines, using IRF5 Rabbit mAb (NBP3-15343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Immunoprecipitation: IRF5 Antibody (6L2B5) [NBP3-15343] - Immunoprecipitation analysis of 600ug extracts of Mouse thymus using 3ug IRF5 antibody (NBP3-15343). Western blot was performed from the immunoprecipitate using IRF5 antibody (NBP3-15343) at a dilition of 1:500.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clone: 6L2B5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IRF5 Antibody (6L2B5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 6-151 of human IRF5 (Q13568). PVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQ
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRF5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100 - 1:500
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for IRF5 Antibody (6L2B5)

• IBD14
• interferon regulatory factor 5
• IRF5
• IRF-5
• SLEB10

Background

IRF5 encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF5