IRAK2 Antibody (600848) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 600848
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

IRAK2 Antibody (600848) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human IRAK2
  Ala2-Asn590
  Accession # O43187
• Specificity: Detects human IRAK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IRAK1, 3, or 4 is observed. In Western blots, less than 25% cross-reactivity with rhIRAK1, 3, and 4 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRAK2 Antibody (600848) [Alexa Fluor® 647]

• interleukin-1 receptor associated kinase-2
• interleukin-1 receptor-associated kinase 2
• interleukin-1 receptor-associated kinase-like 2
• IRAK2
• IRAK-2
• MGC150550

Background

IRAK2 is an approximately 75 kDa, 625 amino acid (aa) member of the IL-1 receptor-associated kinase (IRAK) family. The four IRAKs are ser/thr kinases that regulate signaling from Toll-like and IL-1 receptors. IRAKs contain N-terminal death domains and central kinase domains, but activity of the IRAK2 kinase domain is unknown. IRAK1 provides initial, and IRAK2 sustained, NFkB activation and proinflammatory cytokine production in macrophages, acting downstream of IRAK4. In the region used as an immunogen, human IRAK2 shares 69% and 70% amino acid identity with mouse and rat IRAK2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.