IRAK2 Antibody (600848) [HRP]

• Reactivity: Hu
• Applications: WB
• Clone: 600848
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

IRAK2 Antibody (600848) [HRP] Summary

• Immunogen: E. coli-derived recombinant human IRAK2
  Ala2-Asn590
  Accession # O43187
• Specificity: Detects human IRAK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IRAK1, 3, or 4 is observed. In Western blots, less than 25% cross-reactivity with rhIRAK1, 3, and 4 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRAK2
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for IRAK2 Antibody (600848) [HRP]

• interleukin-1 receptor associated kinase-2
• interleukin-1 receptor-associated kinase 2
• interleukin-1 receptor-associated kinase-like 2
• IRAK2
• IRAK-2
• MGC150550

Background

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK2