Integrin beta 4/CD104 Antibody [Unconjugated]

Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Sheep Anti-Human Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4060) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 4/CD104 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104
  Asn28-Ser710
  Accession # P16144
• Specificity: Detects human Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse Integrin beta 4 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, and rhIntegrin beta 5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGB4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody [Unconjugated]

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 (also known as GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human Integrin  beta 4 ECD shares 88% sequence identity with mouse Integrin beta 4 ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • P16144()