Integrin beta 4/CD104 Antibody

Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4054) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Three specific bands were detected for Integrin beta 4/CD104 at approximately 150-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 4/CD104 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 4/CD104
  Asn29-Cys707
  Accession # NP_001005608
• Specificity: Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Integin beta 4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGB4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 4/CD104 Antibody

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha 6. Mature mouse Integrin beta 4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta 4 shares 88% and 96% aa identity with human and rat beta 4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (AF4054)(2)

Publications using AF4054

• Olga M Viquez Integrin alpha6 maintains the structural integrity of the kidney collecting system Matrix Biol, 2016-12-30;0(0):. 2016-12-30 [PMID: 28043890] (Flow Cytometry, Western Blot, Mouse)
• Eugenia M. Yazlovitskaya, Hui-Yuan Tseng, Olga Viquez, Tianxiang Tu, Glenda Mernaugh, Karen K. McKee et al. Integrin alpha 3 beta 1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt Molecular Biology of the Cell 2015-05-15 [PMID: 25808491] (Western Blot, Transgenic Mouse)

Review for Integrin beta 4/CD104 Antibody (AF4054) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Yongkang Yang WB 12/20/2023

Summary

• Application: Western Blot
• Lot: CCUW0109041

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: ITGB4
• Uniprot:
  • NP_001005608()