Integrin beta 4/CD104 Antibody (CL6973) - BSA Free

Western Blot: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Analysis in human cell line A-431.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human placenta shows strong positivity in basement membrane of trophoblastic cells.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Analysis in human duodenum and liver tissues. Corresponding ITGB4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human duodenum shows moderate membranous immunoreactivity in glandular cells and strong positivity in basement membrane.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Integrin beta 4/CD104 Antibody (CL6973) [NBP2-76525] - Staining of human prostate shows moderate membranous positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL6973
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Integrin beta 4/CD104 Antibody (CL6973) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P16144, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Integrin beta 4/CD104 Antibody (CL6973) - BSA Free

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA); also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB4