Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

Flow Cytometry: Integrin beta 4/CD104 Antibody (439-9B) [Biotin] [NBP1-43599] - Staining of the A549 cell line with 0.125 ug of Rat IgG2b k Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD104 (Integrin BETA 4) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

• Reactivity: Hu, Bv
• Applications: Flow, IHC
• Clone: 439-9B
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: 0.5 mg/ml

Integrin beta 4/CD104 Antibody (439-9B) [Biotin] Summary

• Additional Information: Clone 439-9B was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD104.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGB4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: For WB, use under non-reducing conditions.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 4/CD104 Antibody (439-9B) [Biotin]

• CD104 antigen
• CD104
• GP150
• Integrin beta 4
• integrin beta-4 subunit
• integrin beta-4
• integrin, beta 4
• ITGB4

Background

The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta4. CD104 is a 202 kDa subunit that associates with integrin alpha6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 4/CD104 Antibody (NBP1-43599)(1)

Publication using NBP1-43599

• Graves CL, Harden SW, LaPato M et al. A method for high purity intestinal epithelial cell culture from adult human and murine tissues for the investigation of innate immune function J Immunol Methods 2014-12-01 [PMID: 25193428]
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: ITGB4