Integrin beta 3/CD61 Antibody (EP2417Y)

Product Discontinued

Integrin beta 3/CD61 Antibody (EP2417Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near C-terminus (the cytoplasmic region) of human Integrin beta-3 (CD61 antigen) was used as an immunogen.
• Specificity: This antibody does not detect Integrin Beta-3B or Integrin Beta-3C.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Western Blot 1:1000-2000
• Application Notes: In Western blot this antibody detects a band at approximately 110 kDa.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Integrin beta 3/CD61 Overexpression Lysate

Reactivity Notes

Reacts in Human, Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Integrin beta 3/CD61 Antibody (EP2417Y)

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 3/CD61 Antibody (NB100-79980)(2)

Publications using NB100-79980

• Ruiz de Garibay G, Herranz C, Llorente A et al. Lymphangioleiomyomatosis Biomarkers Linked to Lung Metastatic Potential and Cell Stemness PLoS ONE. 2015-07-14 [PMID: 26167915] (IHC-P, Human)
• L Carramolino, J Fuentes, C Garcia-Andres et al. Platelets Play an Essential Role in Separating the Blood and Lymphatic Vasculatures During Embryonic Angiogenesis. Circ Res; 106 (7): 1197-1201. 2010-01-01 [PMID: 20203303]

Bioinformatics

• Gene Symbol: ITGB3
• Entrez:
  • 3690(Human)
  • 16400(Mouse)
  • 360606(Rat)
• Uniprot:
  • P05106()