Integrin beta 1/CD29 Antibody (TS2/16)

Product Discontinued

Integrin beta 1/CD29 Antibody (TS2/16) Summary

• Immunogen: The immunogen was the full length version of the protein: CD29 (TS2/16)
• Marker: Stem Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 ug/10^6 cells in 100 ul
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, and activation of integrin beta 1. Immunohistochemistry-Frozen and Immunoprecipitation were reported in scientific literature. Use in WB reported in secitific publication PMID: 32573489
• Control:

  Integrin beta 1/CD29 Overexpression Lysate

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 7698991). Expected cross reactivity based on sequence identity: Bovine, Equine. Use in Mouse reported in secitific publication PMID: 32573489

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (TS2/16)

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta 1 is a 130 kD single chain type I glycoprotein, known as integrin beta1, VLA- beta chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. Integrin beta 1 is a member of the integrin family. It is non-covalently associated with integrin alpha1- alpha6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins which include CD29 bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. Integrin beta 1 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NB100-77779)(13)

Publications using NB100-77779

• Doyle AD, Carvajal N, Jin A et al. Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions. Nat Commun. 2015-11-09 [PMID: 26548801] (Human)
• Sanchez-Madrid F, Krensky AM, Ware CF et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3 Proc Natl Acad Sci USA [PMID: 6984191] (IP, Func)
  
  Details:
  Used the FITC form of this antibody.
• Sanchez-Madrid F, Krensky AM, Ware CF et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3 Proc Natl Acad Sci USA (Func, IP)
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NBP1-99998).
• Weber C, Alon R, Moser B, Springer TA. Sequential regulation of alpha4beta1 and alpha5beta1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis J Cell Biol (Func)
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NBP1-99998).
• Schweitzer KS, Jinawath N, Yonescu R et al. IGSF3 mutation identified in patient with severe COPD alters cell function and motility JCI Insight 2020-06-23 [PMID: 32573489] (IP, WB, Mouse)
• Zhang X et al. Am J Physiol Cell Physiol. 301:1017. 2011-01-01 [PMID: 21795522]
• Pillozzi S et al. Blood 117:902. 2011-01-01 [PMID: 21048156]
• Conway RE et al. Mol. Cell. Biol. 26:5310. 2006-01-01 [PMID: 16809768]
• Frank NY, Margaryan A, Huang Y et al. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res 2005-05-15 [PMID: 15899824] (IHC-Fr)
• Porter JC Hogg N. J. Cell Biol. 138:1437. 1997-01-01 [PMID: 9298996]
• Weber C, Alon R, Moser B, Springer TA. Sequential regulation of alpha4beta1 and alpha5beta1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis. J Cell Biol. [PMID: 8769427] (Func, Func)
  
  Details:
  Citation using the FITC form of this antibody.
• Sanchez-Aparicio P et al. J. Cell Biol. 126:271. 1994-01-01 [PMID: 7517944]
• Hemler ME et al. J. Immunol. 132:3011. 1984-01-01 [PMID: 6327814] (IP, IHC-Fr)

FAQs for Integrin beta 1/CD29 Antibody (NB100-77779). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Entrez:
  • 3688(Human)
• Uniprot:
  • P05556()