Integrin beta 1/CD29 Antibody (KMI6) - BSA Free

Western Blot: Integrin beta 1/CD29 Antibody (KMI6) [NBP1-43423] - Balb/c spleen cell lysate (3x10e5 cells/lane) was resolved by SDS-PAGE under non-reducing conditions, immunoblotted with 2 ug/mL Anti-Mouse CD29 Purified and revealed with Anti-Rat IgG HRP.

Product Discontinued

Integrin beta 1/CD29 Antibody (KMI6) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD29.
• Marker: Stem Cell Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.125ug/test
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2.0ug/ml
• Application Notes: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 ug/ml were the antibody will recognize a protein of approximately 120 kD under non-reducing conditions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (KMI6) - BSA Free

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alphabeta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Integrin beta 1/CD29 Antibody (NBP1-43423). (Showing 1 - 2 of 2 FAQ).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.
2. Does this antibody recognize an extracellular epitope of B1-integrin? Should we permeabilize cells to get a signal by flow cytometry?
   • The immunogen is made to the full length protein. As we do not epitope map our antibodies, we are not certain if the antibody will bind to the extracellular or cytoplasmic domain or both. As result, we typically add a permeabilizing step to our flow protocol to ensure staining.

Bioinformatics

• Gene Symbol: ITGB1