Integrin beta 1/CD29 Antibody (DF7)

Product Discontinued

Integrin beta 1/CD29 Antibody (DF7) Summary

• Immunogen: The antibody reacts with the b1 subunit of the integrin protein family.
• Marker: Stem Cell Marker
• Specificity: The antibody reacts with the b1 subunit of the integrin protein family. The monoclonal antibody reacts with an extracellular epitope of the b1 integrin molecule. b1 -Integrins mediate cell-extracellular matrix interactions and share a common b-subunit. Monoclonal antibodies against this b1 -Integrin subunit are applicable in paraffin-embedded and frozen sections, immunoblotting and immunoprecipitation. Numerous studies have shown that b1 -Integrins have a distinct and wide cellular distribution and that there are alterations in malignant cells in their b1-integrin expression. Monoclonal antibody to b1 integrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Wheat germ agglutinin binding glycoproteins of human fibroblasts were used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Immunohistochemical stainings, western blotting and other immunoassays for cellular and tumor biology.Working dilution in western blotting is at least 1:1000.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS solution containing 1.0% w/v BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (DF7)

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Integrin beta 1/CD29 Antibody (NBP1-42318). (Showing 1 - 2 of 2 FAQ).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.
2. I am interested in your antibody # NBP1-42318. However, the primary application I will be using this product for will be immunofluorescence, which is not listed under the recommended applications area of your datasheet. I would appreciate any information you could provide me with about the suitability of this product for IF.
   • Unfortunately we have not validated this antibody for use in IF. However, if you are willing to test this, you would be eligible for our Innovator's Reward Program. Alternatively, you may prefer catalog # NB100-92076 which we have validated for use in IF.

Bioinformatics

• Gene Symbol: ITGB1