Integrin beta 1/CD29 Antibody (BV7) [FITC]

Product Discontinued

Integrin beta 1/CD29 Antibody (BV7) [FITC] Summary

• Immunogen: Human umbilical vein EC
• Marker: Stem Cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Western Blot 1:100-1:2000

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.2 um filtered FITC conjugated antibody solution in PBS and 1.0% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Integrin beta 1/CD29 Antibody (BV7) [FITC]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The monoclonal antibody BV7 recognizes human beta 1 integrin. Beta 1 integrin is a ubiquitously expressed ~89 kDa type I transmembrane protein functioning as receptor when heterodimerized with one alpha subunit. It belongs to the integrin beta chain family consisting of four different genes, encoding multiple beta integrins via alternative splicing. Ligand-recognition depends on the composition of the heterodimer: either collagen, fibronectin, VCAM1, laminin, cytotactin, osteopontin, epiligrin, thrombospondin and CSPG4 can bind to the integrin-complex. Beta 1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with a7, beta 1 integrin regulates cell adhesion and laminin matrix deposition. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. It is involved in promoting endothelial cell motility and angiogenesis. Furthermore, beta 1 integrin plays a mechanistic adhesive role during telophase, and is required for the successful completion of cytokinesis. Upon activation integrins in general, including beta 1 integrin, are known to exhibit global structural rearrangements and exposure of ligand binding sites. beta 1 integrin modulation is of importance in tissue repair and regeneration. In cultured primary hippocampal neurons, astrocytes and tissues, cell surface expression of amyloid beta fibrils (key hallmark of Alzheimer's disease) selectively co-localized with beta 1 integrin. Preincubation of cells with antibodies against beta 1 integrin, as well as a1-integrin, greatly enhanced amyloid beta-induced apoptosis, indicating a protective role for integrins in apoptosis. The monoclonal antibody BV7 does not recognize a5B1 complex and not the cytoplasmic part of the beta 1 subunit. Monoclonal antibody BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.Monoclonal antibody BV7 binds to human beta1 integrin chain present on tumor cells. BV7 is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells. BV7 binds to several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NBP1-60134)(5)

Publications using NBP1-60134

• Bozzo C, Lombardi G, Santoro C, Canonico PL. Involvement of beta(1) integrin in betaAP-induced apoptosis in human neuroblastoma cells Mol Cell Neurosci 2004-02-14 [PMID: 14962735]
• Hofmann G, Bernabei PA, Crociani O et al. HERG K+ channels activation during beta(1) integrin-mediated adhesion to fibronectin induces an up-regulation of alpha(v)beta(3) integrin in the preosteoclastic leukemia cell line FLG 29 J Biol Chem 2000-11-18 [PMID: 11080495]
• Palmieri D, Camardella L, Ulivi V et al. Trimer carboxyl propeptide of collagen I produced by mature osteoblasts is chemotactic for endothelial cells J Biol Chem 2000-08-05 [PMID: 10924500]
• Martel V, Vignoud L, Dupe S et al. Talin controls the exit of the integrin alpha 5 beta 1 from an early compartment of the secretory pathway J Cell Sci 2000-05-12 [PMID: 10806106]
• Martin-Padura I, Bazzoni G, Zanetti A et al. A novel mechanism of colon carcinoma cell adhesion to the endothelium triggered by beta 1 integrin chain J Biol Chem 1994-02-25 [PMID: 7509799]

FAQs for Integrin beta 1/CD29 Antibody (NBP1-60134). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1