Integrin beta 1/CD29 Antibody (12G10) [DyLight 488]

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Product Details

Summary
Reactivity Hu, MdSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
Clone
12G10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

Integrin beta 1/CD29 Antibody (12G10) [DyLight 488] Summary

Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
Epitope
Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.
Localization
Type 1 membrane protein
Marker
Stem Cell Marker
Specificity
NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Integrin beta 1/CD29 Antibody (12G10) [DyLight 488]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NB100-63255G). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-63255AF350
Alexa Fluor 405 NB100-63255AF405
Alexa Fluor 488 NB100-63255AF488
Alexa Fluor 532 NB100-63255AF532
Alexa Fluor 594 NB100-63255AF594
Alexa Fluor 647 NB100-63255AF647
Alexa Fluor 700 NB100-63255AF700
Alexa Fluor 750 NB100-63255AF750
Allophycocyanin NB100-63255APC
Allophycocyanin/Cy7 NB100-63255APCCY7
DyLight 350 NB100-63255UV
DyLight 405 NB100-63255V
DyLight 488 NB100-63255G
DyLight 550 NB100-63255R
DyLight 594 NB100-63255DL594
DyLight 650 NB100-63255C
DyLight 680 NB100-63255FR
DyLight 755 NB100-63255IR
Janelia Fluor 549 NB100-63255JF549
Janelia Fluor 646 NB100-63255JF646
PE NB100-63255PE
PE/Atto594 NB100-63255PEATT594
PE/Cy5.5 NB100-63255PECY55
PE/Cy7 NB100-63255PECY7
PerCP NB100-63255PCP

Additional Integrin beta 1/CD29 Products

Bioinformatics Tool for Integrin beta 1/CD29 Antibody (NB100-63255G)

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Research Areas for Integrin beta 1/CD29 Antibody (NB100-63255G)

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1