Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MHM24 (NBP3-09043) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09043, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: Integrin alpha L/CD11a Antibody (MHM24) - Chimeric [NBP3-09043] - Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of MHM24 (NBP3-09043, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF, B/N
• Clone: MHM24
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody. Clone MHM24 was used by HLDA to establish CD designation.
• Immunogen: Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.
• Specificity: MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGAL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
  
  Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha L/CD11a Antibody (MHM24) - Chimeric - Azide and BSA Free

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. CD11a is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAL