Integrin alpha 5/CD49e Antibody (238307) [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin alpha 5/CD49e Monoclonal Antibody (Catalog # MAB1864, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Integrin alpha 5/CD49e was detected in immersion fixed IMR‑90 human lung fibroblast cell line using Mouse Anti-Human Integrin alpha 5/CD49e Monoclonal Antibody (Catalog # MAB1864) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, Func, ICC/IF
• Clone: 238307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 5/CD49e Antibody (238307) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
  Phe42-Tyr995
  Accession # P08648
• Specificity: Detects human Integrin alpha 5/CD49e.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Activation
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 5/CD49e Antibody (238307) [Unconjugated]

• CD49 antigen-like family member E
• CD49e antigen
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha subunit
• FNRAVLA5A
• Integrin alpha 5
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5
• very late activation protein 5, alpha subunit
• VLA-5 alpha
• VLA-5

Background

The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5/CD49e Antibody (MAB1864)(7)

Publications using MAB1864

• Lechner, A;Jordan, PA;Machado da Cruz, GC;Lamson, J;Gordon, J;Okada, BK;Anderson, K;Chaudhari, R;Rosario, CJ;Mikesell, J;McPhee, SA;Burk, MJ; Overcoming Immune Checkpoint Inhibitor Resistance with Potent, Selective Dual ?v?6/8 Inhibitors Based on Engineered Lasso Peptides Journal of the American Chemical Society 2025-09-10 [PMID: 40891737] (Bioassay, Human)
• MG Jeong, K Zhou, S Park, H An, Y Kwon, Y Chang, DH Kim, SH Ryu Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay Experimental & Molecular Medicine, 2021-02-19;0(0):. 2021-02-19 [PMID: 33603128] (ICC, Human)
• Matteo Bertesi, Sebastian Fantini, Claudia Alecci, Roberta Lotti, Andrea Martello, Sandra Parenti et al. Promoter Methylation Leads to Decreased ZFP36 Expression and Deregulated NLRP3 Inflammasome Activation in Psoriatic Fibroblasts Frontiers in Medicine 2021-01-22 [PMID: 33585499] (Western Blot, Human)
• Verdeny-Vilanova I, Wehnekamp F, Mohan N et al. 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells. J. Cell. Sci. 4/18/2017 [PMID: 28420672]
• Chapple SJ, Keeley TP, Mastronicola D et al. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels. Free Radic. Biol. Med. 12/15/2015 [PMID: 26698668]
• Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. Biomaterials, 2009-04-10;30(20):3415-27. 2009-04-10 [PMID: 19362364] (Flow Cytometry, Human)
• Von Marschall Z, Fisher LW Dentin matrix protein-1 isoforms promote differential cell attachment and migration. J. Biol. Chem., 2008-09-25;283(47):32730-40. 2008-09-25 [PMID: 18819913] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGA5
• Uniprot:
  • P08648()