| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44404 | |||
| BSA Free | NBP2-76520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44402 | |||
| BSA Free | NBP2-76518-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB18642-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44403 | |||
| BSA Free | NBP2-76519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50146-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50147-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52680-0.025mg | |||
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0004.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of A-549 cells, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0002.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (8F2O6) [NBP3-15645] - Analysis of extracts of various cell lines, using Integrin alpha 5/CD49e (ITGA5/CD49e) antibody (NBP3-15645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-8F2O6-Western-Blot-NBP3-15645-img0003.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517303123.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517352792.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734518.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Human placenta using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251734594.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363728.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517344298.jpg)
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Confocal imaging of paraffin-embedded Rat large intestine using Integrin alpha 5/CD49e Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733528.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517363712.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells were intracellularly-stained with Integrin alpha 5/CD49e Rabbit mAb or Rabbit IgG isotype control ,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control .](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-5220251733588.jpg)
![Immunohistochemistry: Integrin alpha 5/CD49e Antibody (8F2O6) [Integrin alpha 5/CD49e] - Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Integrin alpha 5/CD49e Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15645_rabbit-integrin-alpha-5-cd49e-mab-8f2o6-52202517333885.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Western-Blot-NBP2-67883-img0009.jpg "Western Blot: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Western blot analysis of Integrin alpha 5/CD49e on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded mouse uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0007.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of Jurkat cells with Integrin alpha 5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Flow-Cytometry-NBP2-67883-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Staining Integrin alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunocytochemistry-Immunofluorescence-NBP2-67883-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human kidney tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human tonsil tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (JJ08-94) [NBP2-67883] - Analysis of paraffin-embedded human uterus tissue using anti-Integrin alpha 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-JJ08-94-Immunohistochemistry-Paraffin-NBP2-67883-img0006.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0005.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Western-Blot-NBP2-76520-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Analysis in human placenta and skeletal muscle tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human placenta shows strong positivity in apical membranes of trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human liver shows strong positivity in liver sinusoids.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6951) [NBP2-76520] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6951-Immunohistochemistry-Paraffin-NBP2-76520-img0007.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Western-Blot-NBP2-76518-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0005.jpg)
![Western Blot: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Western-Blot-NBP2-76518-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Analysis in human placenta and testis tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6940) [NBP2-76518] - Staining of human endometrium shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6940-Immunohistochemistry-Paraffin-NBP2-76518-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Staining of human prostate shows moderate to strong membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6945-Immunohistochemistry-Paraffin-NBP2-76519-img0005.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Staining of human prostate shows moderate to strong membranous positivity in smooth muscle cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Analysis in human placenta and testis tissues. Corresponding ITGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6945-Immunohistochemistry-Paraffin-NBP2-76519-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Staining of human placenta shows strong apical membrane positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6945-Immunohistochemistry-Paraffin-NBP2-76519-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6945-Immunohistochemistry-Paraffin-NBP2-76519-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody (CL6945) [NBP2-76519] - Staining of human liver shows moderate positivity in liver sinusoids.](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-CL6945-Immunohistochemistry-Paraffin-NBP2-76519-img0003.jpg)
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0002.jpg "Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Separation of human Integrin alpha 5/CD49e positive monocytes (red-filled) from human Integrin alpha 5/CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).")
![Flow Cytometry: Integrin alpha 5/CD49e Antibody (SAM1) [NBP3-18529] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Integrin alpha 5/CD49e (SAM1) purified antibody (concentration in sample 1.7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-5-CD49e-Antibody-SAM1-Flow-Cytometry-NBP3-18529-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52680-img0003.jpg "Immunohistochemistry-Paraffin: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - N87 human gastric tumor xenograft immunostained with isotope control antibody (left panel) or 1:200 dilution of Integrin alpha 5 beta 1 antibody (right panel). Arrows indicate DAB immunoreactivity. Red scale bar = 10 microns. Image from verified customer review.")
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Flow-Cytometry-NBP2-52680-img0002.jpg)
![Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-5-beta-1-Antibody-M200-Volociximab-Chimeric-Immunofluorescence-NBP2-52680-img0001.jpg)
![Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric - Azide and BSA Free [NBP2-52680] - Abundance of two potential Hla receptors & of caveolin-1 as a potential pore-stabilizing factor.A: Representative examples of immune fluorescence assays using epifluorescence microscopy (nuclei counterstained using DAPI) that were performed on 16HBE14o-, S9 or A549 cells grown on coverslips using antibodies against ADAM10, the alpha 5 beta 1 integrin or against caveolin-1. Staining appearing in red represents specific labelling of the respective proteins. Scale bars: 10 μm. B: Semi-quantitative determination of primary & secondary antibody-mediated fluorescence in suspended individual cells by flow cytometry. During flow cytometry, the fluorescence of the antibody-tagged proteins per cell was measured & the respective medians of the detected peaks were used for calculating the means ± S.D. for the biological replicates (n = 4, each). Individual means were tested for significant differences using Student’s t-test or Welch’s t-test: * = p ≤ 0.05, ** = p ≤ 0.01 or *** = p ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32470006), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-52680_rabbit-monoclonal-integrin-alpha-5-beta-1-antibody-m200-volociximab-chimeric-310202416114751.jpg)
