| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | M200 (Volociximab) |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | DyLight 550 |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Heterodimeric alpha 5 Beta 1 Fc fusion protein |
| Specificity | The antibody binds to human (alpha)5(beta)1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ITGA5 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) [DyLight 550]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) [DyLight 550]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-52680AF350 | |
| Alexa Fluor 405 | NBP2-52680AF405 | |
| Alexa Fluor 488 | NBP2-52680AF488 | |
| Alexa Fluor 532 | NBP2-52680AF532 | |
| Alexa Fluor 594 | NBP2-52680AF594 | |
| Alexa Fluor 647 | NBP2-52680AF647 | |
| Alexa Fluor 700 | NBP2-52680AF700 | |
| Alexa Fluor 750 | NBP2-52680AF750 | |
| Allophycocyanin | NBP2-52680APC | |
| Allophycocyanin/Cy7 | NBP2-52680APCCY7 | |
| Biotin | NBP2-52680B | |
| DyLight 350 | NBP2-52680UV | |
| DyLight 405 | NBP2-52680V | |
| DyLight 488 | NBP2-52680G | |
| DyLight 550 | NBP2-52680R | |
| DyLight 650 | NBP2-52680C | |
| DyLight 680 | NBP2-52680FR | |
| DyLight 755 | NBP2-52680IR | |
| FITC | NBP2-52680F | |
| HRP | NBP2-52680H | |
| Janelia Fluor 549 | NBP2-52680JF549 | |
| Janelia Fluor 646 | NBP2-52680JF646 | |
| PE | NBP2-52680PE | |
| PE/Atto594 | NBP2-52680PEATT594 | |
| PE/Cy5.5 | NBP2-52680PECY55 | |
| PE/Cy7 | NBP2-52680PECY7 | |
| PerCP | NBP2-52680PCP |
Diseases for Integrin alpha 5 beta 1 Antibody (NBP2-52680R)Discover more about diseases related to Integrin alpha 5 beta 1 Antibody (NBP2-52680R).
| Pathways for Integrin alpha 5 beta 1 Antibody (NBP2-52680R)View related products by pathway.
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PTMs for Integrin alpha 5 beta 1 Antibody (NBP2-52680R)Learn more about PTMs related to Integrin alpha 5 beta 1 Antibody (NBP2-52680R).
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| Gene Symbol | ITGA5 |
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/images2/Integrin-alpha-L-CD11a-Antibody-(MEM-25)-Flow-Cytometry-NB500-328-img0004.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Analysis using the PE conjugate of NB500-328. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.](http://images.novusbio.com/images2/Integrin-alpha-L-CD11a-Antibody-(MEM-25)-Flow-Cytometry-NB500-328-img0002.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Staining pattern of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/images2/Integrin-alpha-L-CD11a-Antibody-(MEM-25)-Flow-Cytometry-NB500-328-img0003.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining in human placenta and liver tissues. Corresponding ITGA6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Integrin-alpha-6-CD49f-Antibody-Immunohistochemistry-Paraffin-NBP1-85747-img0022.jpg)
![Western Blot: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/Integrin-alpha-6-CD49f-Antibody-Western-Blot-NBP1-85747-img0016.jpg)
![Western Blot: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/images2/Integrin-alpha-6-CD49f-Antibody-Western-Blot-NBP1-85747-img0010.jpg)
![Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/images2/Integrin-alpha-2-CD49b-Antibody-(SN0752)-Western-Blot-NBP2-67691-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Integrin-alpha-2-CD49b-Antibody-(SN0752)-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse stomach tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Integrin-alpha-2-CD49b-Antibody-(SN0752)-Immunohistochemistry-Paraffin-NBP2-67691-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Image from product review by verified customer.](http://images.novusbio.com/images2/CD11b-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89474-img0010.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/CD11b-Antibody-Dual-RNAscope-ISH-IHC-NB110-89474-img0025.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (http://www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD11b-Antibody-Immunohistochemistry-NB110-89474-img0024.jpg)
![Western Blot: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/Integrin-alpha-1-CD49a-Antibody-(CL7207)-Western-Blot-NBP2-76478-img0007.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Staining of human placenta shows strong membranous positivity in blood vessels and loose connective tissue of chorionic villi.](http://images.novusbio.com/images2/Integrin-alpha-1-CD49a-Antibody-(CL7207)-Immunohistochemistry-Paraffin-NBP2-76478-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7207) [NBP2-76478] - Analysis in human prostate and skeletal muscle tissues. Corresponding ITGA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Integrin-alpha-1-CD49a-Antibody-(CL7207)-Immunohistochemistry-Paraffin-NBP2-76478-img0002.jpg)