Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

Flow Cytometry: Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) [NBP1-43283] - Staining of C57BL/6 splenocytes with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse Integrin alpha 4 beta 7 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.

• Reactivity: Mu
• Applications: Flow
• Clone: DATK32
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free Summary

• Immunogen: The immunogen for this antibody was LPAM1.
• Epitope: The monoclonal antibody DATK32 recognizes a conformational epitope on the heterodimer, integrin alpha 4 beta 7 (LPAM-1).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This DATK32 antibody has been tested tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 4 beta 7/LPAM-1 Antibody (DATK32) - BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49d/beta7
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4 beta 7
• integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• LPAM1
• LPAM-1
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha

Background

The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit. In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4
• Entrez:
  • 3676(Human)
  • 3695(Human)
  • 16401(Mouse)
  • 16421(Mouse)
• Uniprot:
  • P13612()
  • P26011()
  • Q00651(Mouse)