Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated]

Images

 
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) ...read more
Integrin alpha 3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
IA3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated] Summary

Immunogen
Human milk epithelial cell line
Specificity
Detects human Integrin alpha 3/CD49c.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGA3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1345 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 3/CD49c Antibody (IA3) [Unconjugated]

  • antigen identified by monoclonal J143
  • CD49 antigen-like family member C
  • CD49c antigen
  • CD49c
  • FLJ34631
  • FLJ34704
  • FRP-2
  • Galactoprotein B3
  • GAP-B3
  • GAPB3CD49C
  • Integrin alpha 3
  • integrin alpha-3
  • integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
  • ITGA3
  • MSK18
  • VCA-2
  • very late activation protein 3 receptor, alpha-3 subunit
  • VL3A
  • VLA-3 subunit alpha
  • VLA3a

Background

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).
  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 3/CD49c Antibody (MAB1345)(3)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1345U
Alexa Fluor 405 FAB1345V
Alexa Fluor 488 FAB1345G
Alexa Fluor 594 FAB1345T
Alexa Fluor 647 FAB1345R
Alexa Fluor 700 FAB1345N
Alexa Fluor 750 FAB1345S
Biotin BAM1345
Fluorescein FAB1345F

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Bioinformatics

Gene Symbol ITGA3