HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) ...read more
Integrin alpha 3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room ...read more
very late activation protein 3 receptor, alpha-3 subunit
VLA-3 subunit alpha
(Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily,
of cell membrane adhesion molecules (1-3). Integrins are
nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha -
and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The
alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only
associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to
generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain
and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy
chain contains seven 60 aa repeats that fold into a propeller-like
structure (7). Sequences involving the first three repeats are
associated with ligand binding (1). The light chain has two cytoplasmic
alternate splice forms. The A form cytoplasmic domain is 52 aa, while
the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9,
CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it
to bind to additional factors (8).
Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
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