Integrin alpha 3/CD49c Antibody (IA3) [Fluorescein]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a3/CD49c Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1345F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: IA3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Integrin alpha 3/CD49c Antibody (IA3) [Fluorescein] Summary

• Immunogen: Human milk epithelial cell line
• Specificity: Detects human Integrin alpha 3/CD49c.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 3/CD49c Antibody (IA3) [Fluorescein]

• antigen identified by monoclonal J143
• CD49 antigen-like family member C
• CD49c antigen
• CD49c
• FLJ34631
• FLJ34704
• FRP-2
• Galactoprotein B3
• GAP-B3
• GAPB3CD49C
• Integrin alpha 3
• integrin alpha-3
• integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• ITGA3
• MSK18
• VCA-2
• very late activation protein 3 receptor, alpha-3 subunit
• VL3A
• VLA-3 subunit alpha
• VLA3a

Background

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1 - 3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130 - 150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 3/CD49c Antibody (FAB1345F)(2)

Publications using FAB1345F

• L Das, JMC Gard, R Prekeris, RB Nagle, C Morrissey, BS Knudsen, CK Miranti, AE Cress Novel Regulation of Integrin Trafficking by Rab11-FIP5 in Aggressive Prostate Cancer Mol. Cancer Res., 2018-05-14;0(0):. 2018-05-14 [PMID: 29759989] (Flow Cytometry, Human)
• Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. Biomaterials, 2009-04-10;30(20):3415-27. 2009-04-10 [PMID: 19362364] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGA3