INSIG-1 Antibody [DyLight 488]

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

INSIG-1 Antibody [DyLight 488] Summary

• Immunogen: A synthetic peptide representing an internal region of the human INSIG-1 protein (between residues 1-100) according to Swiss-Prot# O15503.
• Localization: ER Membrane
• Specificity: This is specific for Insig1 but not Insig2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INSIG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: This INSIG-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at ~30 kDa representing the Insig1 protein. In ICC/IF positive staining was localized to the ER membrane in HepG2 cells.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for INSIG-1 Antibody [DyLight 488]

• CL6
• CL-6
• INSIG-1 membrane protein
• INSIG-1
• insulin induced gene 1
• insulin-induced gene 1 protein
• MGC1405

Background

Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSIG1