INO80E Overexpression Lysate (Native)

Images

 
Western Blot: INO80E Overexpression Lysate (Adult Normal) [NBL1-08834] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INO80E.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

INO80E Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208292 and based on accession number NM_173618. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens INO80 complex subunit E (INO80E), mRNA.
Gene
INO80E

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for INO80E

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for INO80E Overexpression Lysate (Native)

  • CCDC95
  • coiled-coil domain containing 95
  • Coiled-coil domain-containing protein 95
  • FLJ00079
  • FLJ90652
  • INO80 complex subunit E

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF7817
Species: Hu, Mu, Rt
Applications: WB
NBP2-30524
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-40845
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP
NBP2-19939
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP1-40343
Species: Hu
Applications: WB, IHC-P, IP
NBP1-91269
Species: Hu, Mu
Applications: ICC/IF (-), IHC (-), WB
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NBP1-87109
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00008031-M04
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
MAB4184
Species: Hu, Mu
Applications: WB, IP, ICC
NB100-56519
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl
NB100-57521
Species: Hu, Mu
Applications: WB, IP
NBP1-83228
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-250
Species: Hu
Applications: WB, ChIP, IHC, IHC-P, IP, PLA
NBP1-87835
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB120-13888
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC-P, CyTOF-ready, IHC-WhMt
NBP1-51593
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-266
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-28731
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-44092
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for INO80E Lysate (NBL1-08834) (0)

There are no publications for INO80E Lysate (NBL1-08834).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for INO80E Lysate (NBL1-08834) (0)

There are no reviews for INO80E Lysate (NBL1-08834). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for INO80E Lysate (NBL1-08834) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional INO80E Products

Bioinformatics Tool for INO80E Lysate (NBL1-08834)

Discover related pathways, diseases and genes to INO80E Lysate (NBL1-08834). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for INO80E Lysate (NBL1-08834)

View related products by pathway.

Blogs on INO80E

There are no specific blogs for INO80E, but you can read our latest blog posts.

Customers Who Bought This Also Bought

INO80E Antibody
NBP1-86578

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our INO80E Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol INO80E