KAT1 Antibody

Images

 
Western Blot: KAT1 Antibody [NBP1-87835] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-HAT1 antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: KAT1 Antibody [NBP1-87835] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining in human lymph node and pancreas tissues using anti-HAT1 antibody. Corresponding HAT1 RNA-seq data are presented for the same tissues.
Western Blot: KAT1 Antibody [NBP1-87835] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: KAT1 Antibody [NBP1-87835] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

KAT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KFKINKQHARRVYEILRLLVTDMSDAEQYRSYRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLA
Specificity
Specificity of human, mouse, rat KAT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
KAT1 Protein (NBP1-87835PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for KAT1 Antibody

  • EC 2.3.1.48
  • histone acetyltransferase 1
  • histone acetyltransferase type B catalytic subunit
  • KAT1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HAT1