Importin beta/KPNB1 Antibody

Western Blot: Importin beta/KPNB1 Antibody [NB100-94993] - Detection of Human and Mouse KPNB1 on HeLa whole cell lysate using NB100-94993.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Importin beta/KPNB1 Antibody Summary

• Description: Novus Biologicals Rabbit Importin beta/KPNB1 Antibody (NB100-94993) is a polyclonal antibody validated for use in WB and IP. Anti-Importin beta/KPNB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 826 and 876 of human karyopherin (importin) beta 1 using the numbering given in entry NP_002256.2 (GeneID 3837).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin beta/KPNB1 Antibody

• IMB1
• Impnb
• Importin 1
• importin 90
• Importin beta
• importin beta-1 subunit
• importin subunit beta-1
• importin-90
• IPO1
• IPOB
• karyopherin (importin) beta 1
• Karyopherin subunit beta-1
• KPNB1
• MGC2155
• MGC2156
• MGC2157
• NTF97
• NTF97Pore targeting complex 97 kDa subunit
• Nuclear factor p97
• PTAC97

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin beta/KPNB1 Antibody (NB100-94993)(1)

Publication using NB100-94993

• Li J, Zhao J, Xu S et al. Antiviral activity of a purine synthesis enzyme reveals a key role of deamidation in regulating protein nuclear import Sci Adv 2019-10-01 [PMID: 31633017] (WB, Human)

Bioinformatics

• Gene Symbol: KPNB1
• Entrez:
  • 3837(Human)
  • 16211(Mouse)
• Uniprot:
  • Q14974(Human)