Importin alpha 2/KPNA2 Antibody (682239) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Importin a2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Importin a2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 682239
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Importin alpha 2/KPNA2 Antibody (682239) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Importin alpha 2/KPNA2
  Ser2-Asp132
  Accession # P52292
• Specificity: Detects human Importin alpha 2/KPNA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human KPNA1, A3, A4, A5, or B1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KPNA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Importin alpha 2/KPNA2 Antibody (682239) [Unconjugated]

• Importin alpha 1
• Importin alpha 2
• importin-alpha-P1
• IPOA1
• karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Karyopherin subunit alpha-2
• KPNA2
• Pendulin
• QIP2
• QIP2importin alpha 2
• RAG cohort 1
• RAG cohort protein 1
• RCH1
• RCH1importin subunit alpha-2
• SRP1
• SRP1alpha
• SRP1-alpha

Background

Importin alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, or RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin beta domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human Importin alpha 2 shares 94% aa identity with mouse Importin alpha 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin alpha 2/KPNA2 Antibody (MAB6207)(4)

Publications using MAB6207

• I Andreu, I Granero-Mo, NR Chahare, K Clein, M Molina-Jor, AEM Beedle, A Elosegui-A, JF Abenza, L Rossetti, X Trepat, B Raveh, P Roca-Cusac Mechanical force application to the nucleus regulates nucleocytoplasmic transport Nature Cell Biology, 2022-06-09;24(6):896-905. 6/9/2022 [PMID: 35681009]
• CC Chang, CJ Chen, C Grauffel, YC Pien, C Lim, SY Tsai, KC Hsia Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α Nat Commun, 2019-09-20;10(1):4307. 2019-09-20 [PMID: 31541088] (ICC, Immunoprecipitation, Human)
• Gasset-Rosa F, Lu S, Yu H et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death Neuron 3/5/2019 [PMID: 30853299]
• Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent PLoS ONE, 2016-08-31;11(8):e0162033. 2016-08-31 [PMID: 27579771] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: KPNA2
• Uniprot:
  • P52292(Human)