Importin beta/KPNB1 Antibody - BSA Free

Western Blot: Importin beta/KPNB1 Antibody [NB100-79806] - Importin beta Antibody [NB100-79806] - Detection of KapB1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-KapB1 antibody BL1964 (Cat. No. NB100-79806) used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was unsuccessful when using rabbit anti-KapB1 antibody BL1962 (Cat. No. NB100-79805) at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Importin beta/KPNB1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 825 and the C-terminus (residue 876) of human Karyopherin (importin) beta 1 using the numbering given in entry NP_002256.2 (GeneID 3837).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:15000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin beta/KPNB1 Antibody - BSA Free

• IMB1
• Impnb
• Importin 1
• importin 90
• Importin beta
• importin beta-1 subunit
• importin subunit beta-1
• importin-90
• IPO1
• IPOB
• karyopherin (importin) beta 1
• Karyopherin subunit beta-1
• KPNB1
• MGC2155
• MGC2156
• MGC2157
• NTF97
• NTF97Pore targeting complex 97 kDa subunit
• Nuclear factor p97
• PTAC97

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin beta/KPNB1 Antibody (NB100-79806)(1)

Publication using NB100-79806

• Kim DY, Sub YJ, Kim HY et al. LRRC6 regulates biogenesis of motile cilia by aiding FOXJ1 translocation into the nucleus Cell communication and signaling : CCS 2023-06-16 [PMID: 37328841] (Immunoprecipitation)

Bioinformatics

• Gene Symbol: KPNB1
• Entrez:
  • 3837(Human)
  • 16211(Mouse)
• Uniprot:
  • NP_002256.2(Human)