Importin-9 Antibody

Images

 
Genetic Strategies: Knockout Validated: Importin-9 Antibody [NB100-56499] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. ...read more
Western Blot: Importin-9 Antibody [NB100-56499] - Western blot analysis of Importin-9 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using NB100-56499 at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, RMSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Importin-9 Antibody Summary

Immunogen
A portion of amino acids 850-950 of human Importin-9 was used as the immunogen.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, _b, _c), mouse (isoforms 1 and 2), rat, dog and rhesus monkey, 88% homologous in chicken, 83% homologous in opossum, and 78% in bovine.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IPO9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Knockout Validated 1 ug/ml
  • Western Blot 1-3 ug/ml
Control
HeLa Whole Cell Lysate

Reactivity Notes

Predicted to react with Bovine, Chicken and Opossum.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Importin-9 Antibody

  • DKFZp761M1547
  • FLJ10402
  • Imp9IMP9
  • importin 9
  • importin-9
  • KIAA1192
  • Ran-binding protein 9
  • ranBP9

Background

Official Gene Symbol: IPO9 Gene ID: 55705 (human) Gene Map Locus: 1q32.1 (human) Importin-9 is a novel member of highly conserved Karyopherin Super family. Karyopherins are transport receptors consisting of an N-terminal RanGTP-binding motif and mediate the transport of protein and RNA across the nucleus and cytoplasm. These proteins interact directly with nuclear pore complex and shuttle continuously between the nucleus and cytoplasm. Importin-9 is a RAN-GTP binding protein and mediates the nuclear import of individual core histones and TBP. Reports suggest Importin-9 also interacts with subunits of PP2A (protein phosphatase 2A) and aids in nuclear import.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IPO9
Entrez
Uniprot