Importin-13 Antibody - BSA Free

Immunohistochemistry: Importin-13 Antibody - BSA Free [NBP3-46464] - Immunohistochemistry of paraffin-embedded human gliomas using (Importin-13 antibody) at dilution of 1:50

Western Blot: Importin-13 Antibody - BSA Free [NBP3-46464] - Human brain tissue were subjected to SDS PAGE followed by western blot with (Importin-13 antibody) at dilution of 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Importin-13 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Importin-13 Antibody - BSA Free (NBP3-46464) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: importin 13. (Uniprot# O94829)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IPO13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Importin-13 Antibody - BSA Free

• IMP13
• importin 13
• importin-13
• KAP13
• karyopherin 13
• Karyopherin-13
• KIAA0724karyopherin-13
• LGL2
• Ran binding protein 13
• Ran-binding protein 13
• RanBP13
• RANBP13late gestation lung 2

Background

Importin-13 functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. Expressed in fetal brain, heart, intestine and kidney. Belongs to the importin beta family. Contains 14 HEAT repeats. Contains 1 importin N-terminal domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IPO13