Western Blot: Importin-13 Antibody [NBP1-31508] - Analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate. B: IPO13 transfected lysate. 7.5% SDS PAGE, antibody ...read more
Immunocytochemistry/ Immunofluorescence: Importin-13 Antibody [NBP1-31508] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Importin-13 Antibody [NBP1-31508] - Paraffin-embedded CL1-5 xenograft, using antibody at 1:100 dilution.
Western Blot: Importin-13 Antibody [NBP1-31508] - IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody. The precipitated IPO13 was detected by antibody diluted at 1:1000.
Immunoprecipitation, Immunofluorescence, Western blot. The usefulness of this product in other applications has not been determined.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
108 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-31508 in the following applications:
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. Expressed in fetal brain, heart, intestine and kidney. Belongs to the importin beta family. Contains 14 HEAT repeats. Contains 1 importin N-terminal domain.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.