IMPA2 Overexpression Lysate

Images

 
Western Blot: IMPA2 Overexpression Lysate (Adult Normal) [NBL1-11983] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IMPA2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IMPA2 Overexpression Lysate Summary

Description

IMPA2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200063

Accession#: NM_014214

Protein Tag: C-MYC/DDK
Gene
IMPA2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
31.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IMPA2 Overexpression Lysate

  • EC 3.1.3.25
  • IMP 2
  • IMP.18P
  • IMPase 2
  • inosine monophosphatase 2
  • inositol monophosphatase 2 variant 1
  • inositol monophosphatase 2 variant 2
  • inositol monophosphatase 2
  • inositol(myo)-1(or 4)-monophosphatase 2
  • Inositol-1(or 4)-monophosphatase 2
  • Myo-inositol monophosphatase A2

Background

IMPA2, also known as Inositol monophosphatase 2, has a 288 amino acid isoform that is 31 kDa and a short 261 amino acid that is 29 kDa, catalyzes the dephosphoylration of inositol monophosphate and plays an important role in phosphatidylinositol signaling, can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates, and serves as possible pharmacological target for lithium Li+ action in brain. Studies are being performed on the relationship of this protein to bipolar disorder, mood disorder, seizures, schizophrenia, febrile seizures, bipolar affective disorder, neisseria meningitides, pneumonia, tuberculosis, neuronitis, schizophrenia and leukemia. The protein has been shown to interact with HERPUD1, TNFRSF1H, INPP1, ISYNA1, and PLCG1 in inositol phosphate metabolism, metabolic pathways and phosphatidylinositol signaling system pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-83107
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DBD00
Species: Hu
Applications: ELISA
NBP2-94071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB110-40773
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-32225
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-77681
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
396-HB/CF
Species: Hu
Applications: BA
NB300-105
Species: Hu, Mu, Rb, Rt
Applications: IHC, IHC-P, IP, WB
NB300-106
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, WB
NBP2-47415
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14040
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-92892
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
201-LB/CF
Species: Hu
Applications: BA
DFN00
Species: Hu
Applications: ELISA
AF5129
Species: Hu
Applications: WB
AF2408
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
NBP1-89559
Species: Hu
Applications: IHC, IHC-P, WB
210-TA
Species: Hu
Applications: BA
NBL1-11983
Species: Hu
Applications: WB

Publications for IMPA2 Lysate (NBL1-11983) (0)

There are no publications for IMPA2 Lysate (NBL1-11983).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IMPA2 Lysate (NBL1-11983) (0)

There are no reviews for IMPA2 Lysate (NBL1-11983). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IMPA2 Lysate (NBL1-11983) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IMPA2 Products

Bioinformatics Tool for IMPA2 Lysate (NBL1-11983)

Discover related pathways, diseases and genes to IMPA2 Lysate (NBL1-11983). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IMPA2 Lysate (NBL1-11983)

Discover more about diseases related to IMPA2 Lysate (NBL1-11983).
 

Pathways for IMPA2 Lysate (NBL1-11983)

View related products by pathway.

PTMs for IMPA2 Lysate (NBL1-11983)

Learn more about PTMs related to IMPA2 Lysate (NBL1-11983).
 

Research Areas for IMPA2 Lysate (NBL1-11983)

Find related products by research area.

Blogs on IMPA2

There are no specific blogs for IMPA2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IMPA2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol IMPA2