GNAL Antibody - BSA Free

Western Blot: GNAL Antibody [NBP1-32225] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry: GNAL Antibody [NBP1-32225] - Mouse brain tissue. Antibody at 1:500. Selective and strong signal in the striatum. Image submitted by a verified customer review.

Western Blot: GNAL Antibody [NBP1-32225] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-32225] - NCI-N87 xenograft. GNAL antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

GNAL antibody detects GNAL protein at cell membrane by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNAL stained by GNAL antibody (NBP1-32225) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GNAL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GNAL Antibody - BSA Free (NBP1-32225) is a polyclonal antibody validated for use in IHC and WB. Anti-GNAL Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GNAL. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Chicken (95%), Xenopus (90%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNAL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (87%), Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GNAL Antibody - BSA Free

• Adenylate cyclase-stimulating G alpha protein, olfactory type
• guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory typ
• guanine nucleotide binding protein (G protein), alpha activating activitypolypeptide, olfactory type
• guanine nucleotide binding protein (G protein), alpha stimulating activitypolypeptide, olfactory type
• guanine nucleotide-binding protein G(olf) subunit alpha

Background

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP, and most obviously regulate the activity of the best studied effectors. The Gs subfamily of Galpha subunits includes two closely related proteins, Galpha s and Galpha olf, which, respectively, stimulate adenylate cyclase and mediate response to olfactory stimuli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GNAL Antibody (NBP1-32225)(1)

Publication using NBP1-32225

• Kim By, Park Jy, Cho Kj, Bae Jh Effects of Urban Particulate Matter on the Olfactory System in a Mouse Model American journal of rhinology & allergy 2021-07-08 [PMID: 34236242]

Review for GNAL Antibody (NBP1-32225) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Fuming Zhou IHC Mouse 05/14/2018

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: 3040732

Comments

• Comments: immunohistochemistry on brain tissue.
  NBP1-32225 diluted at 1:500, leading to a strong signal with high selectivity, see the image.

Bioinformatics

• Gene Symbol: GNAL