IMPA1 Antibody (984603) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB98901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IMPA1/IMP1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 984603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IMPA1 Antibody (984603) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human IMPA/IMPA1 Peptide
  Accession # P29218
• Specificity: Detects human IMPA/IMPA1 Peptide in direct ELISAs. Detects human, mouse, and rat IMPA/IMPA1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IMPA1 Antibody (984603) [Unconjugated]

• EC 3.1.3.25
• IMP 1
• IMP
• IMP1
• IMPA
• IMPA1
• IMPase 1
• inositol monophosphatase 1
• inositol(myo)-1(or 4)-monophosphatase 1
• Inositol-1(or 4)-monophosphatase 1
• Lithium-sensitive myo-inositol monophosphatase A1
• MRT59
• Myo-Inositol Monophosphatase 1

Background

IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P29218()