ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated]


Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRB2/CD85d/ILT4 Monoclonal Antibody (Catalog # MAB2078) more
Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready

Order Details

ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human LILRB2/CD85d/ILT4
Accession # ACT64556
Detects human LILRB2/CD85d/ILT4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT6, rhILT7, rhILT11, rhLIR6 or rhLIR8 is observed.
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.2-0.8 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
MAB2078 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated]

  • CD85 antigen-like family member D
  • CD85d antigen
  • CD85d
  • Ig-like transcript 4
  • ILT4
  • ILT-4
  • ILT4CD85d
  • Immunoglobulin-like transcript 4
  • Leukocyte immunoglobulin-like receptor 2
  • leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 2
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB2
  • LIR2
  • LIR2CD85D
  • LIR-2subfamily A (with TM domain), member 6
  • member 2
  • MIR10
  • Monocyte/macrophage immunoglobulin-like receptor 10


The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1‑3). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) (4). Human ILT4 is produced as a 598 amino acid (aa) precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain. The ECD contains four Ig-like domains, and the cytoplasmic domain contains three immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64 % aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5‑9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1 (4, 6). Activation of ILT4 inhibits signaling through Fc gamma RI (4) and Fc epsilon RI (6) and causes DC to become tolerogenic by downregulation of costimulatory molecules (10, 11). ILT4 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo (10‑12).

  1. Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7. 
  2. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317. 
  3. Hunt, J.S. et al. (2005) FASEB J. 19:681. 
  4. Finger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. 
  5. Borges, L. et al. (1997) J. Immunol. 159:5192. 
  6. Colonna, M. et al. (1998) J. Immunol. 160:3096. 
  7. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. 100:8856.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  10. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  11. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
  12. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILT4/CD85d/LILRB2 Antibody (MAB2078)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Neutralization.

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Showing Publications 1 - 7 of 7.
Publications using MAB2078 Applications Species
X Chen, A Gao, F Zhang, Z Yang, S Wang, Y Fang, J Li, J Wang, W Shi, L Wang, Y Zheng, Y Sun ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation Theranostics, 2021;11(7):3392-3416. 2021 [PMID: 33537094] (ICC, Human) ICC Human
S Tomi?, N Ili?, V Kokol, A Gruden-Mov, D Mihajlovi?, M Beki?, L Sofroni?-M, M ?oli?, D Vu?evi? Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells Int J Nanomedicine, 2018;13(0):6941-6960. 2018 [PMID: 30464452] (Neutralization, Human) Neutralization Human
Giles J, Shaw J, Piper C, Wong-Baeza I, McHugh K, Ridley A, Li D, Lenart I, Antoniou AN, DiGleria K, Kuroki K, Maenaka K, Bowness P, Kollnberger S HLA-B27 homodimers and free H chains are stronger ligands for leukocyte Ig-like receptor B2 than classical HLA class I. J. Immunol., 2012;188(12):6184-93. 2012 [PMID: 22593621] (Neutralization, Human) Neutralization Human
Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. J. Immunol., 2011;186(12):6701-9. 2011 [PMID: 21543643] (Flow Cytometry, Human) Flow Cytometry Human
Wenink MH, Santegoets KC, Roelofs MF, Huijbens R, Koenen HJ, van Beek R, Joosten I, Meyer-Wentrup F, Mathsson L, Ronnelid J, Adema GJ, Bonvini E, Koenig S, van den Berg WB, van Riel PL, Radstake TR The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease. J. Immunol., 2009;183(7):4509-20. 2009 [PMID: 19734236] (Flow Cytometry, Human) Flow Cytometry Human
Lichterfeld M, Kavanagh DG, Williams KL, Moza B, Mui SK, Miura T, Sivamurthy R, Allgaier R, Pereyra F, Trocha A, Feeney M, Gandhi RT, Rosenberg ES, Altfeld M, Allen TM, Allen R, Walker BD, Sundberg EJ, Yu XG A viral CTL escape mutation leading to immunoglobulin-like transcript 4-mediated functional inhibition of myelomonocytic cells. J. Exp. Med., 2007;204(12):2813-24. 2007 [PMID: 18025130] (Neutralization, Human) Neutralization Human
Masuda A, Nakamura A, Maeda T, Sakamoto Y, Takai T Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation. J. Exp. Med., 2007;204(4):907-20. 2007 [PMID: 17420263] (Flow Cytometry, Human) Flow Cytometry Human

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Alexa Fluor 350 FAB2078U
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Alexa Fluor 700 FAB2078N
Alexa Fluor 700 FAB2078N
Alexa Fluor 750 FAB2078S
Allophycocyanin FAB2078A
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Biotin FAB2078B
DyLight 350 FAB2078D
DyLight 405 FAB2078E
DyLight 488 FAB2078K
DyLight 550 FAB2078L
DyLight 594 FAB2078M
DyLight 650 FAB2078W
DyLight 680 FAB2078Y
DyLight 755 FAB2078Z
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Janelia Fluor 646 FAB2078J
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Gene Symbol LILRB2