ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated]

Images

 
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRB2/CD85d/ILT4 Monoclonal Antibody (Catalog # MAB2078) ...read more
Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
287219
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB2/CD85d/ILT4
Gly24-His458
Accession # ACT64556
Specificity
Detects human LILRB2/CD85d/ILT4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ILT1, rhILT2, rhILT3, rhILT6, rhILT7, rhILT11, rhLIR6 or rhLIR8 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LILRB2
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 0.2-0.8 ug/mL
Publications
Read Publications using
MAB2078 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated]

  • CD85 antigen-like family member D
  • CD85d antigen
  • CD85d
  • Ig-like transcript 4
  • ILT4
  • ILT-4
  • ILT4CD85d
  • Immunoglobulin-like transcript 4
  • Leukocyte immunoglobulin-like receptor 2
  • leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform
  • leukocyte immunoglobulin-like receptor subfamily B member 2
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB2
  • LIR2
  • LIR2CD85D
  • LIR-2subfamily A (with TM domain), member 6
  • member 2
  • MIR10
  • MIR10LILRA6
  • Monocyte/macrophage immunoglobulin-like receptor 10

Background

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1‑3). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC) (4). Human ILT4 is produced as a 598 amino acid (aa) precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain. The ECD contains four Ig-like domains, and the cytoplasmic domain contains three immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64 % aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5‑9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1 (4, 6). Activation of ILT4 inhibits signaling through Fc gamma RI (4) and Fc epsilon RI (6) and causes DC to become tolerogenic by downregulation of costimulatory molecules (10, 11). ILT4 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo (10‑12).

  1. Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7. 
  2. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317. 
  3. Hunt, J.S. et al. (2005) FASEB J. 19:681. 
  4. Finger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. 
  5. Borges, L. et al. (1997) J. Immunol. 159:5192. 
  6. Colonna, M. et al. (1998) J. Immunol. 160:3096. 
  7. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. 100:8856.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  10. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  11. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133.
  12. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB20172
Species: Hu
Applications: WB, CyTOF-reported, Neut
AF2425
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-43123
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB6364
Species: Hu
Applications: Flow, CyTOF-ready
NB120-6405
Species: Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB1806
Species: Hu
Applications: WB, Flow, CyTOF-ready
H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-25255
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
MAB12251
Species: Hu
Applications: Flow, CyTOF-ready

Publications for ILT4/CD85d/LILRB2 Antibody (MAB2078)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Neutralization.


Filter By Application
Flow Cytometry
(3)
Neutralization
(3)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB2078 Applications Species
S Tomi?, N Ili?, V Kokol, A Gruden-Mov, D Mihajlovi?, M Beki?, L Sofroni?-M, M ?oli?, D Vu?evi? Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells Int J Nanomedicine, 2018;13(0):6941-6960. 2018 [PMID: 30464452] (Neutralization, Human) Neutralization Human
Giles J, Shaw J, Piper C, Wong-Baeza I, McHugh K, Ridley A, Li D, Lenart I, Antoniou AN, DiGleria K, Kuroki K, Maenaka K, Bowness P, Kollnberger S HLA-B27 homodimers and free H chains are stronger ligands for leukocyte Ig-like receptor B2 than classical HLA class I. J. Immunol., 2012;188(12):6184-93. 2012 [PMID: 22593621] (Neutralization, Human) Neutralization Human
Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. J. Immunol., 2011;186(12):6701-9. 2011 [PMID: 21543643] (Flow Cytometry, Human) Flow Cytometry Human
Wenink MH, Santegoets KC, Roelofs MF, Huijbens R, Koenen HJ, van Beek R, Joosten I, Meyer-Wentrup F, Mathsson L, Ronnelid J, Adema GJ, Bonvini E, Koenig S, van den Berg WB, van Riel PL, Radstake TR The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease. J. Immunol., 2009;183(7):4509-20. 2009 [PMID: 19734236] (Flow Cytometry, Human) Flow Cytometry Human
Lichterfeld M, Kavanagh DG, Williams KL, Moza B, Mui SK, Miura T, Sivamurthy R, Allgaier R, Pereyra F, Trocha A, Feeney M, Gandhi RT, Rosenberg ES, Altfeld M, Allen TM, Allen R, Walker BD, Sundberg EJ, Yu XG A viral CTL escape mutation leading to immunoglobulin-like transcript 4-mediated functional inhibition of myelomonocytic cells. J. Exp. Med., 2007;204(12):2813-24. 2007 [PMID: 18025130] (Neutralization, Human) Neutralization Human
Masuda A, Nakamura A, Maeda T, Sakamoto Y, Takai T Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation. J. Exp. Med., 2007;204(4):907-20. 2007 [PMID: 17420263] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for ILT4/CD85d/LILRB2 Antibody (MAB2078) (0)

There are no reviews for ILT4/CD85d/LILRB2 Antibody (MAB2078). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ILT4/CD85d/LILRB2 Antibody (MAB2078) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB2078U
Alexa Fluor 405 FAB2078V
Alexa Fluor 488 FAB2078G
Alexa Fluor 594 FAB2078T
Alexa Fluor 647 FAB2078R
Alexa Fluor 700 FAB2078N
Alexa Fluor 700 FAB2078N
Alexa Fluor 750 FAB2078S
Allophycocyanin FAB2078A
Allophycocyanin FAB2078A
Phycoerythrin FAB2078P
Phycoerythrin FAB2078P

Additional ILT4/CD85d/LILRB2 Products

Bioinformatics Tool for ILT4/CD85d/LILRB2 Antibody (MAB2078)

Discover related pathways, diseases and genes to ILT4/CD85d/LILRB2 Antibody (MAB2078). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ILT4/CD85d/LILRB2 Antibody (MAB2078)

Discover more about diseases related to ILT4/CD85d/LILRB2 Antibody (MAB2078).
 

Pathways for ILT4/CD85d/LILRB2 Antibody (MAB2078)

View related products by pathway.

PTMs for ILT4/CD85d/LILRB2 Antibody (MAB2078)

Learn more about PTMs related to ILT4/CD85d/LILRB2 Antibody (MAB2078).

Blogs on ILT4/CD85d/LILRB2

There are no specific blogs for ILT4/CD85d/LILRB2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILT4/CD85d/LILRB2 Antibody (287219) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol LILRB2
Uniprot