Home » ILT3/CD85k/LILRB4 » ILT3/CD85k/LILRB4 Antibodies » ILT3/CD85k/LILRB4 Antibody [Unconjugated]

ILT3/CD85k/LILRB4 Antibody [Unconjugated]

 (1 Publication)
 See All Related
Submit a Review

Images

 
There are currently no images for ILT3/CD85k/LILRB4 Antibody (AF2425).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2425
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2425-SP
100% Guarantee on every product

ILT3/CD85k/LILRB4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3
Pro17-His257
Accession # ABM86208
Specificity
Detects human LILRB4/CD85k/ILT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) ILT5 is observed and 10% cross-reactivity with rhILT4 and rhILT2 is observed.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LILRB4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publication using
AF2425 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT3/CD85k/LILRB4 Antibody [Unconjugated]

  • CD85 antigen-like family member K
  • CD85k antigen
  • CD85k
  • HM18
  • ILT3
  • ILT-3
  • ILT3CD85K
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB4
  • LIR5
  • LIR5LILRB5
  • LIR-5subfamily B, member 4
  • member 4

Background

ILT3, also known as leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) and leukocyte immunoglobulin-like receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ILT4/CD85d/LILRB2
Antibody (287219) [Un ...
MAB2078
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRB2/CD85d/ILT4 Monoclonal Antibody (Catalog # MAB2078) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (35 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB2/CD85d/ILT4 Monoclonal Antibody (Catalog # MAB2078). The ND50 is typically0.2-0.8 µg/mL.
(6 Publications)
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
ILT2/CD85j/LILRB1
Antibody (292319) [Un ...
MAB20172
Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND50 is typically 0.025‑0.15 µg/mL.
(5 Publications)
Species: Hu
Applications: WB, CyTOF-reported, Neut
Doublecortin
Antibody (3E1)
NBP1-92684
Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
CD4 Antibody
NBP1-19371
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
(8 Reviews)
(19 Publications)
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CD8 Antibody
(53-6.7)
NBP1-49045
Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(2 Reviews)
(13 Publications)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
(3 Reviews)
(1 Publication)
Species: Hu
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
HLA DQ Antibody
(SPV-L3)
NBP2-45041
HLA DQ Antibody (SPV-L3)
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
B7-2/CD86 Antibody
(BU63)
NBP2-25208
Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.
Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Surface staining of CD86 on 10^6 PBMC using 0.25 ug of CD86 antibody.
(7 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
B7-1/CD80 Antibody
(62N3G8)
NBP2-25255
Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.
Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.
(2 Reviews)
(1 Publication)
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
FoxP3 Antibody
NB100-39002
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
(1 Review)
(13 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP
Coagulation Factor
XIV/Protein C Antibod ...
NBP2-66987
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
APC Antibody
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.
Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
CD28 Antibody
(37407) [Unconjugated
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.2-0.6 μg/mL.
(26 Publications)
Species: Hu
Applications: WB, AgAct
Thromboxane
synthase Antibody (OT
NBP2-02710
Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.
Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.
(1 Publication)
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Thymidylate
Synthase Antibody (SP
NBP2-34390
Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on HEK293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free format of this antibody.
(1 Review)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Cav1.2 Antibody
(S57-46)
NBP1-22439
Western Blot: Cav1.2 Antibody (S57-46) [NBP1-22439] - analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57-46) [NBP1-22439] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.
Immunohistochemistry: Cav1.2 Antibody (S57-46) [NBP1-22439] - Human hippocampus tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.
(3 Publications)
Species: Hu, Mu, Rt, Ha, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
ILT3/CD85k/LILRB4
Antibody [Unconjugate ...
AF2425
ILT3/CD85k/LILRB4 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for ILT3/CD85k/LILRB4 Antibody (AF2425)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


Filter By Application
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for ILT3/CD85k/LILRB4 Antibody (AF2425) (0)

There are no reviews for ILT3/CD85k/LILRB4 Antibody (AF2425). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ILT3/CD85k/LILRB4 Antibody (AF2425) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2425
Learn about Custom Antibody Labeling

Additional ILT3/CD85k/LILRB4 Products

Bioinformatics Tool for ILT3/CD85k/LILRB4 Antibody (AF2425)

Discover related pathways, diseases and genes to ILT3/CD85k/LILRB4 Antibody (AF2425). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ILT3/CD85k/LILRB4 Antibody (AF2425)

Discover more about diseases related to ILT3/CD85k/LILRB4 Antibody (AF2425).
 

Pathways for ILT3/CD85k/LILRB4 Antibody (AF2425)

View related products by pathway.

PTMs for ILT3/CD85k/LILRB4 Antibody (AF2425)

Learn more about PTMs related to ILT3/CD85k/LILRB4 Antibody (AF2425).

Blogs on ILT3/CD85k/LILRB4

There are no specific blogs for ILT3/CD85k/LILRB4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
CTLA-4 [Unconjugated]
7268-CT-100
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
CD4 Antibody
NBP1-19371

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ILT3/CD85k/LILRB4 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol LILRB4
Uniprot