HLA G Antibody (MEM-G/1)

Images

 
Western Blot: HLA G Antibody (MEM-G/1) [NB500-302] - Using the Biotin direct conjugate analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.
Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains ...read more
Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1B - first-trimester placenta (paraffin-embedded sections)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
MEM-G/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HLA G Antibody (MEM-G/1) Summary

Immunogen
Denatured bacterially expressed recombinant human HLA-G heavy chain.
Localization
Cell Membrane
Specificity
The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-G
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Immunohistochemistry-Paraffin sections Incubation: 1 h at RT Staining technique: Standard ABC technique Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
Publications
Read Publications using
NB500-302 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27849611)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for HLA G Antibody (MEM-G/1)

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • major histocompatibility complex, class I, G
  • MHC class I antigen G
  • MHC-G

Background

HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA G Antibody (NB500-302)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Mouse
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Blogs on HLA G.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-G

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