IL12B Antibody

Western Blot: IL12B Antibody [NB100-61661] - (0.3ug/ml) staining of Human Liver (A), Skin (B) and Tonsil (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IL12B Antibody Summary

• Immunogen: Peptide with sequence C-QGKSKREKKDRVFTD corresponding to internal region according to NP_002178.2.
• Epitope: QGKSKREKKDRVFTD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL12B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:16000
  • Western Blot 0.1ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human liver lysates (calculated MW of 37.2 kDa band according to NP_002178.2).
• Control:

  IL12B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL12B Antibody

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2IL12, subunit p40

Background

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Over expression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL12B Antibody (NB100-61661)(1)

Publication using NB100-61661

• Sheth K, De A, Nolan B et al. Heat shock protein 27 inhibits apoptosis in human neutrophils. J Surg Res 2001-07-01 [PMID: 11421614]

Bioinformatics

• Gene Symbol: IL12B
• Entrez:
  • 3593(Human)
• Uniprot:
  • NP_002178.2(Human)