IL12B Antibody (I-1A4) - BSA Free

ELISA: IL12B Antibody (I-1A4) [NB100-64693] - Direct ELISA showing cross reactivity of Mouse anti Human IL-12 with porcine IL-12. Antigen coated at 3ugml in ELISA coating buffer (BUF030) and Goat anti Mouse Ig (STAR87P) as tertiary detection reagent

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: I-1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IL12B Antibody (I-1A4) - BSA Free Summary

• Description: Novus Biologicals Mouse IL12B Antibody (I-1A4) - BSA Free (NB100-64693) is a monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human IL-12
• Specificity: Recognizes the p40 subunit of human interleukin-12, and binds to both the free subunit and the p35/p40 heterodimer. The reacts with both stimulated and unstimulated monocytes in immunohistology.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000

Reactivity Notes

Predicted cross-reactivities: Porcine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL12B Antibody (I-1A4) - BSA Free

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2IL12, subunit p40

Background

IL12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL12 is a potent stimulator of NK cells. IL12 therapy has been suggested as a method of enhancing cytocidal anti tumor immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL12B
• Entrez:
  • 3593(Human)
• Uniprot:
  • P29460()
  • P29460()