IL-9 Antibody (623153) [Allophycocyanin]

204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." > 204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." title="Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 APC-conjugated Monoclonal Antibody (Catalog # IC209A) or (B) Mouse IgG2B Isotype Control Antibody (IC0041A) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol." />

Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (<a class=

• Reactivity: Hu
• Applications: Flow
• Clone: 623153
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IL-9 Antibody (623153) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
  Gln19-Ile144
  Accession # P15248
• Specificity: Detects human IL-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat IL-9 or recombinant mouse IL-9 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-9 Antibody (623153) [Allophycocyanin]

• Cytokine P40
• HP40
• IL9
• IL-9
• IL-9homolog of mouse T cell and mast cell growth factor 40
• interleukin 9
• interleukin-9
• p40 cytokine
• p40 T-cell and mast cell growth factor
• P40
• T-cell growth factor P40

Background

Interleukin-9 (IL-9) is a 28-30 kDa monomeric glycosylated polypeptide that belongs to the IL-7/IL-9 family of proteins. Although multiple cell types are reported to express IL-9, including Th17 cells, eosinophils, basophils, NK T cells and mast cells, the two cell types possibly most central to the process are Th9 and ILC-2 (or innate lymphoid cell type 2) cells. IL-9 supports the growth of multiple hematopoietic cell types including germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. It also promotes IgE production, the activation and chemoattraction of mast cells, mucus production by epithelium, Th17 cell proliferation and TGF-beta production by dendritic cells. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-9 Antibody (IC209A)(1)

Publication using IC209A

• R Talotta, A Berzi, A Doria, A Batticciot, MC Ditto, F Atzeni, P Sarzi-Putt, D Trabattoni The Immunogenicity of Branded and Biosimilar Infliximab in Rheumatoid Arthritis According to Th9-Related Responses Int J Mol Sci, 2017-10-12;18(10):. 2017-10-12 [PMID: 29023386] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL9
• Uniprot:
  • P15248()