IL-6 Antibody [Biotin]

IL-6 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF406) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (brown; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-6 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL-6
  Phe25-Leu211
  Accession # P08505
• Specificity: Detects mouse IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross‑reactivity with recombinant human IL-6, recombinant rat IL-6, and recombinant porcine IL-6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-6 Antibody (Catalog # MAB406)
  ELISA Detection: Mouse IL-6 Biotinylated Antibody (Catalog # BAF406)
  Standard: Recombinant Mouse IL-6 (Catalog # 406-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6 Antibody [Biotin]

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6 Antibody (BAF406)(29)

Publications using BAF406

• Hong, J;Son, M;Sin, J;Kim, H;Chung, DK; Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation Nutrients 2023-11-09 [PMID: 38004123] (ELISA Detection, Mouse)
• S Van Belle, A de Lange, H Tomes, R Lucas, V Naidoo, JV Raimondo Peripheral Taenia infection increases immunoglobulins in the central nervous system International journal for parasitology, 2021-03-20;51(8):685-692. 2021-03-20 [PMID: 33753094] (Mouse)
• HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway Arthritis Research & Therapy, 2020-09-23;22(1):222. 2020-09-23 [PMID: 32972460] (Human)
• DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway Mol Med Rep, 2019-11-19;0(0):. 2019-11-19 [PMID: 31746359] (ELISA Development, Mouse)
• JM Ho, NH Ducich, NK Nguyen, MR Opp Acute sleep disruption- and high-fat diet-induced hypothalamic inflammation are not related to glucose tolerance in mice Neurobiol Sleep Circadian Rhythms, 2018-01-01;4(0):1-9. 1/1/2018 [PMID: 29732438]
• Daniel Busch, Amar Kapoor, Pia Rademann, Frank Hildebrand, Soheyl Bahrami, Christoph Thiemermann et al. Delayed activation of PPAR-beta /δ improves long-term survival in mouse sepsis: effects on organ inflammation and coagulation Innate Immunity 5/1/2018 [PMID: 29697010]
• T Mitani, T Takaya, N Harada, S Katayama, R Yamaji, S Nakamura, H Ashida Theophylline suppresses interleukin-6 expression by inhibiting glucocorticoid receptor signaling in pre-adipocytes Arch. Biochem. Biophys., 2018-04-04;646(0):98-106. 2018-04-04 [PMID: 29625124] (ELISA Detection, Mouse)
• JR Lee, I Appelmann, C Miething, TO Shultz, D Ruderman, D Kim, P Mallick, SW Lowe, SX Wang Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors Theranostics, 2018-02-03;8(5):1389-1398. 2/3/2018 [PMID: 29507628]
• Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection J. Leukoc. Biol., 2017-10-10;0(0):. 2017-10-10 [PMID: 29018150] (ELISA Development (Detection), Mouse)
• R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Mouse)
• S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling J. Biol. Chem, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280243] (Western Blot, Human)
• Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (ELISA Development (Detection), Mouse)
• Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J KIM-1-mediated phagocytosis reduces acute injury to the kidney. J Clin Invest, 2015-03-09;125(4):1620-36. 2015-03-09 [PMID: 25751064] (ELISA Development (Detection), Mouse)
• Dominic De Nardo, Larisa I. Labzin, Hajime Kono, Reiko Seki, Susanne V. Schmidt, Marc Beyer et al. High density lipoprotein mediates anti-inflammatory transcriptional reprogramming of macrophages via the transcriptional repressor ATF3 Nature Immunology 2/1/2014 [PMID: 24317040]
• Junkins R, MacNeil A, Wu Z, McCormick C, Lin T Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections. J Immunol, 2013-04-15;190(10):5178-86. 2013-04-15 [PMID: 23589609] (ELISA Development (Detection), Mouse)
• Michael Timm, Lasse Saaby, Lise Moesby, Erik Wind Hansen Considerations regarding use of solvents in in vitro cell based assays Cytotechnology 10/1/2013 [PMID: 23328992]
• Meng Y, Efimova EV, Hamzeh KW Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine. Mol. Ther., 2012-02-14;20(5):1046-55. 2012-02-14 [PMID: 22334019] (IHC-P, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Mouse)
• Osuchowski MF, Connett J, Welch K, Granger J, Remick DG Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis. Crit. Care Med., 2009-05-01;37(5):1567-73. 2009-05-01 [PMID: 19325479] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-03-13;11(4):524-30. 2009-03-13 [PMID: 19286469] (ELISA Development, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA Development, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse)
• An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006-03-10;106(3):418-24. 2006-03-10 [PMID: 16530364] (ELISA Development, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse)
• Fredj S, Bescond J, Louault C, Delwail A, Potreau D Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation. J. Cell. Physiol., 2005-08-01;204(2):428-36. 2005-08-01 [PMID: 15717324] (ELISA Development, Mouse)
• Pechova J, StÄ•pánová G, Kovar L, Kopecky J Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitol., 2002-01-01;49(2):153-9. 2002-01-01 [PMID: 12194488] (ELISA Development, Mouse)

FAQs for IL-6 Antibody (BAF406). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P08505()