IL-6 Antibody (OTI3G9) - Azide and BSA Free

Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 antibody (2G3) at 1:2000

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.

Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6. Positive lysates LY424617 (100ug) and LC424617 (20ug).

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Anti-IL-6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL-6.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human colon tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human colon tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Kidney tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-6 Antibody (OTI3G9) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:50000
• Theoretical MW: 23.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IL-6 Antibody (OTI3G9) - Azide and BSA Free

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IL-6 Antibody (NBP2-71027). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6