IL-6 Antibody (OTI3G9) - Azide and BSA Free

Images

 
Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.
Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - IL-6 antibody (3G9) at 1:500000 + recombinant human IL-6 IL-6 antibody (2G3) at 1:2000.
Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Anti-IL-6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human colon tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human colon tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Kidney tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI3G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

IL-6 Antibody (OTI3G9) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IL6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
Theoretical MW
23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for IL-6 Antibody (OTI3G9) - Azide and BSA Free

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HGF
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-22471
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP2-27406
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
NBP2-71027
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for IL-6 Antibody (NBP2-71027) (0)

There are no publications for IL-6 Antibody (NBP2-71027).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-6 Antibody (NBP2-71027) (0)

There are no reviews for IL-6 Antibody (NBP2-71027). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-6 Antibody (NBP2-71027). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71027AF350
Alexa Fluor 405 NBP2-71027AF405
Alexa Fluor 488 NBP2-71027AF488
Alexa Fluor 532 NBP2-71027AF532
Alexa Fluor 594 NBP2-71027AF594
Alexa Fluor 647 NBP2-71027AF647
Alexa Fluor 700 NBP2-71027AF700
Alexa Fluor 750 NBP2-71027AF750
Biotin NBP2-71027B
DyLight 350 NBP2-71027UV
DyLight 405 NBP2-71027V
DyLight 488 NBP2-71027G
DyLight 550 NBP2-71027R
DyLight 594 NBP2-71027DL594
DyLight 650 NBP2-71027C
DyLight 680 NBP2-71027FR
DyLight 755 NBP2-71027IR
FITC NBP2-71027F
HRP NBP2-71027H
Janelia Fluor 549 NBP2-71027JF549
Janelia Fluor 646 NBP2-71027JF646

Additional IL-6 Products

Bioinformatics Tool for IL-6 Antibody (NBP2-71027)

Discover related pathways, diseases and genes to IL-6 Antibody (NBP2-71027). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-6 Antibody (NBP2-71027)

Discover more about diseases related to IL-6 Antibody (NBP2-71027).
 

Pathways for IL-6 Antibody (NBP2-71027)

View related products by pathway.

PTMs for IL-6 Antibody (NBP2-71027)

Learn more about PTMs related to IL-6 Antibody (NBP2-71027).
 

Research Areas for IL-6 Antibody (NBP2-71027)

Find related products by research area.

Blogs on IL-6. Showing 1-10 of 11 blog posts - Show all blog posts.

COVID-19-Related Neurological Problems: Uncovering the Mechanisms
By Jamshed Arslan, Pharm D, PhDThere is good news and bad news related to COVID-19, a disease caused by SARS-COV-2 infection. Good news first: the vast majority of otherwise healthy individuals remain asymptomatic...  Read full blog post.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.

Gut-brain axis: microbiota influence behavior and mental well-being
By Jennifer Sokolowski, MD, PhD.Gut-microbiome interactionsOn and in our bodies, microbes outnumber our cells by about ten-to-one. Studies have revealed that the microbiome influences neurogenesis, cognition, and...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-6 Antibody (OTI3G9) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol IL6