Human IL-5 ELISA Kit (Colorimetric)

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Human IL-5 ELISA Kit [NBP1-91274] - Data for Human IL-5 Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.5 pg/mL

Order Details

Human IL-5 ELISA Kit (Colorimetric) Summary

Description
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5.
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-5 positive serum. There was no crossreactivity detected.
Standard Curve Range
7.8 - 500 pg/mL
Sensitivity
1.5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 6.80%
Intra-Assay
CV% < 6.60%
Spike Recovery
74%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Plate Covers
  2. Aluminium pouch(es) with a human IL-5 Standard curve (coloured)
  3. Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IL-5, Biotin-Conjugate (anti-IL-5 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  4. Sample Diluent (Use when an external predilution of the samples is needed)
  5. Stop Solution (1M Phosphoric acid)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Alternate Names for Human IL-5 ELISA Kit (Colorimetric)

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for IL-5 ELISA Kit (NBP1-91274). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol IL5