Human IL-5 ELISA Kit (Colorimetric) Summary
| Description |
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. |
| Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-5 positive serum. There was no crossreactivity detected. |
| Standard Curve Range |
7.8 - 500 pg/mL |
| Sensitivity |
1.5 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 6.80% |
| Intra-Assay |
CV% < 6.60% |
| Spike Recovery |
74% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL5 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Plate Covers
- Aluminium pouch(es) with a human IL-5 Standard curve (coloured)
- Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human IL-5, Biotin-Conjugate (anti-IL-5 monoclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
- Sample Diluent (Use when an external predilution of the samples is needed)
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
|
Alternate Names for Human IL-5 ELISA Kit (Colorimetric)
Background
The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-5 ELISA Kit (NBP1-91274). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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